This is critical when analyzing brain structures. This will provide an unambiguous reference for left and right. It may be helpful to notch the side of your organ of interest before sectioning. Vishnus character faces a problem, however, as he has to try hard to win the approval of his girlfriends father. The romantic comedy stars Vishnu and Catherine Tresa as a loving couple. Frozen tissue is very easy to tear.Ĥ) Use landmarks to identify the ‘handedness’ of your tissue. With Meera Krishnan, Saranya Ponvannan, Vijay Sethupathi, T.R. Using a 96 well tissue culture plate is a great way to keep your sections in order.ģ) Handle your tissue carefully. Before an investigator begins sectioning tissue for stereology it may be helpful to practice sectioning techniques on unimportant tissue whose loss will not affect the study.ġ) Try to collect every section that comes off your cryostat, vibratome, etc… This will mean more tissue to work with later.Ģ) Maintain the sequential order of your tissue. The most likely time to damage or lose tissue is during sectioning. During dissection care must be taken not to puncture, cut or otherwise damage the organ in question, as doing so could damage deep structures that are the target of analysis. Damage occurring during tissue preparation may lead to tissue that is not of the quality needed and thus great care must be taken during organ harvesting, as well as during the sectioning of tissue. The collection and preparation of sample tissue is important. Performing an unbiased stereological study begins long before the investigator is at the microscope.
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